منابع مشابه
Regulation of Raf-1 and Raf-1 mutants by Ras-dependent and Ras-independent mechanisms in vitro.
The serine/threonine kinase Raf-1 functions downstream from Ras to activate mitogen-activated protein kinase kinase, but the mechanisms of Raf-1 activation are incompletely understood. To dissect these mechanisms, wild-type and mutant Raf-1 proteins were studied in an in vitro system with purified plasma membranes from v-Ras- and v-Src-transformed cells (transformed membranes). Wild-type (His)6...
متن کاملDeficient mismatch repair and RAS mutation in colorectal carcinoma patients: a retrospective study in Eastern China
Objectives To investigate the frequency and prognostic role of deficient mismatch repair (dMMR) and RAS mutation in Chinese patients with colorectal carcinoma. Methods Clinical and pathological information from 813 patients were reviewed and recorded. Expression of mismatch repair proteins was tested by immunohistochemistry. Mutation analyses for RAS gene were performed by real-time polymeras...
متن کاملRaf Inhibitors Target Ras Spatiotemporal Dynamics
BACKGROUND The lateral segregation of Ras proteins into transient plasma membrane nanoclusters is essential for high-fidelity signal transmission by the Ras mitogen-activated protein kinase (MAPK) cascade. In this spatially constrained signaling system, the dynamics of Ras nanocluster assembly and disassembly control MAPK signal output. RESULTS We show here that BRaf inhibitors paradoxically ...
متن کاملDual function of Ras in Raf activation.
The small guanine nucleotide binding protein p21(Ras) plays an important role in the activation of the Raf kinase. However, the precise mechanism by which Raf is activated remains unclear. It has been proposed that the sole function of p21(Ras )in Raf activation is to recruit Raf to the plasma membrane. We have used Drosophila embryos to examine the mechanism of Raf (Draf) activation in the com...
متن کاملIrinotecan and mismatch repair deficiency.
We read the recent publications of Ychou et al. [1, 2] on ‘adjuvant’ irinotecan-based chemotherapy in high-risk localized colorectal cancer (CRC) and after resection of liver-confined metastatic disease with great interest. In contrast to the improved outcome provided by oxaliplatin-based adjuvant treatment of early-stage disease, there is now a body of evidence showing that the addition of iri...
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ژورنال
عنوان ژورنال: Genome Biology
سال: 2002
ISSN: 1465-6906
DOI: 10.1186/gb-spotlight-20020830-01